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Effects of NS5806 on I to channel-related gene and protein expression. ( A – C ) Quantification of the mRNA levels of Kv4.2 ( A ), <t>KChIP2</t> ( B ) and DPP6 ( C ). GAPDH served as an internal control (n = 9 in each group). ( D ) Representative immunoblots. Original blots are presented in Supplementary Figure . ( E – G ) Summary data of Kv4.2 ( E ), KChIP2 ( F ) and DPP6 ( G ) protein levels. GAPDH served as an internal control (n = 5 in each group). * P < 0.05, ** P < 0.01 versus the Sham + Vehicle group. # P < 0.05, ## P < 0.01 versus TAC + Vehicle.
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Effects of NS5806 on I to channel-related gene and protein expression. ( A – C ) Quantification of the mRNA levels of Kv4.2 ( A ), KChIP2 ( B ) and DPP6 ( C ). GAPDH served as an internal control (n = 9 in each group). ( D ) Representative immunoblots. Original blots are presented in Supplementary Figure . ( E – G ) Summary data of Kv4.2 ( E ), KChIP2 ( F ) and DPP6 ( G ) protein levels. GAPDH served as an internal control (n = 5 in each group). * P < 0.05, ** P < 0.01 versus the Sham + Vehicle group. # P < 0.05, ## P < 0.01 versus TAC + Vehicle.

Journal: Scientific Reports

Article Title: The Kv4 potassium channel modulator NS5806 attenuates cardiac hypertrophy in vivo and in vitro

doi: 10.1038/s41598-024-70962-x

Figure Lengend Snippet: Effects of NS5806 on I to channel-related gene and protein expression. ( A – C ) Quantification of the mRNA levels of Kv4.2 ( A ), KChIP2 ( B ) and DPP6 ( C ). GAPDH served as an internal control (n = 9 in each group). ( D ) Representative immunoblots. Original blots are presented in Supplementary Figure . ( E – G ) Summary data of Kv4.2 ( E ), KChIP2 ( F ) and DPP6 ( G ) protein levels. GAPDH served as an internal control (n = 5 in each group). * P < 0.05, ** P < 0.01 versus the Sham + Vehicle group. # P < 0.05, ## P < 0.01 versus TAC + Vehicle.

Article Snippet: Primary antibodies against Kv4.2 (1:500, 75–016, NeuroMab, USA), KChIP2 (1:500, DF14620, Affinity, China), DPP6 (1:200, APC-146, Alomone, Israel) and GAPDH (1:10,000, 10494-1-AP, Proteintech, China) were incubated at 4 °C overnight.

Techniques: Expressing, Control, Western Blot